1
|
/**
|
2
|
* Copyright (C) 2007 EDIT
|
3
|
* European Distributed Institute of Taxonomy
|
4
|
* http://www.e-taxonomy.eu
|
5
|
*
|
6
|
* The contents of this file are subject to the Mozilla Public License Version 1.1
|
7
|
* See LICENSE.TXT at the top of this package for the full license terms.
|
8
|
*/
|
9
|
package eu.etaxonomy.cdm.model.molecular;
|
10
|
|
11
|
|
12
|
import java.net.URI;
|
13
|
import java.net.URISyntaxException;
|
14
|
import java.util.HashSet;
|
15
|
import java.util.Set;
|
16
|
|
17
|
import javax.persistence.Column;
|
18
|
import javax.persistence.Entity;
|
19
|
import javax.persistence.FetchType;
|
20
|
import javax.persistence.ManyToMany;
|
21
|
import javax.persistence.ManyToOne;
|
22
|
import javax.persistence.OneToMany;
|
23
|
import javax.persistence.Transient;
|
24
|
import javax.xml.bind.annotation.XmlAccessType;
|
25
|
import javax.xml.bind.annotation.XmlAccessorType;
|
26
|
import javax.xml.bind.annotation.XmlAttribute;
|
27
|
import javax.xml.bind.annotation.XmlElement;
|
28
|
import javax.xml.bind.annotation.XmlElementWrapper;
|
29
|
import javax.xml.bind.annotation.XmlIDREF;
|
30
|
import javax.xml.bind.annotation.XmlRootElement;
|
31
|
import javax.xml.bind.annotation.XmlSchemaType;
|
32
|
import javax.xml.bind.annotation.XmlTransient;
|
33
|
import javax.xml.bind.annotation.XmlType;
|
34
|
|
35
|
import org.apache.log4j.Logger;
|
36
|
import org.hibernate.annotations.Cascade;
|
37
|
import org.hibernate.annotations.CascadeType;
|
38
|
import org.hibernate.envers.Audited;
|
39
|
import org.hibernate.search.annotations.IndexedEmbedded;
|
40
|
import org.springframework.beans.factory.annotation.Configurable;
|
41
|
|
42
|
import eu.etaxonomy.cdm.common.CdmUtils;
|
43
|
import eu.etaxonomy.cdm.model.common.AnnotatableEntity;
|
44
|
import eu.etaxonomy.cdm.model.media.Media;
|
45
|
import eu.etaxonomy.cdm.model.reference.Reference;
|
46
|
import eu.etaxonomy.cdm.model.term.DefinedTerm;
|
47
|
import eu.etaxonomy.cdm.model.term.TermType;
|
48
|
|
49
|
/**
|
50
|
* Alignment of multiple {@link SingleRead single sequences} to a consensus sequence.
|
51
|
* This sequence is a part of (or the complete) DNA sequences of the related {@link DnaSample DNA Sample},
|
52
|
* while
|
53
|
*
|
54
|
* <BR>This class holds information about both the combining process of
|
55
|
* {@link SingleRead single sequences} to one consensus sequence
|
56
|
* ({@link #getSingleReads() singleReads} , {@link #getContigFile() contigFile} )
|
57
|
* as well as sequence related information.
|
58
|
* The later includes the {@link #getConsensusSequence() sequence string} itself,
|
59
|
* important genetic information about the DNA that has been sequenced
|
60
|
* ({@link #getDnaMarker() marker} , {@link #getHaplotype()} haplotype) as well as
|
61
|
* registration information ({@link #getGeneticAccessionNumber() genetic accession number} ),
|
62
|
* citations, and barcoding information ({@link #getBoldProcessId() BOLD-id},
|
63
|
* {@link #getBarcodeSequencePart() barcode sequence}, ...).
|
64
|
*
|
65
|
* @author m.doering
|
66
|
* @since 08-Nov-2007 13:06:51
|
67
|
* @author a.mueller
|
68
|
* @updated 11-Jul-2013
|
69
|
*/
|
70
|
@XmlAccessorType(XmlAccessType.FIELD)
|
71
|
@XmlType(name = "Sequence", propOrder = {
|
72
|
"dnaSample",
|
73
|
"consensusSequence",
|
74
|
"isBarcode",
|
75
|
"barcodeSequencePart",
|
76
|
"dnaMarker",
|
77
|
"geneticAccessionNumber",
|
78
|
"boldProcessId",
|
79
|
"haplotype",
|
80
|
"contigFile",
|
81
|
"singleReadAlignments",
|
82
|
"citations"
|
83
|
})
|
84
|
@XmlRootElement(name = "Sequencing")
|
85
|
@Entity
|
86
|
@Audited
|
87
|
@Configurable
|
88
|
//@Table(name="Sequence", indexes = { @Index(name = "sequenceTitleCacheIndex", columnList = "titleCache") })
|
89
|
public class Sequence
|
90
|
extends AnnotatableEntity
|
91
|
implements Cloneable{
|
92
|
|
93
|
private static final long serialVersionUID = 8298983152731241775L;
|
94
|
private static final Logger logger = Logger.getLogger(Sequence.class);
|
95
|
|
96
|
//TODO move to cdmlib-ext?
|
97
|
private static final String GENBANK_BASE_URI = "http://www.ncbi.nlm.nih.gov/nuccore/%s";
|
98
|
private static final String EMBL_BASE_URI = "http://www.ebi.ac.uk/ena/data/view/%s";
|
99
|
private static final String DDBJ_BASE_URI = "http://getentry.ddbj.nig.ac.jp/getentry/na/%s/?filetype=html";
|
100
|
private static final String BOLD_BASE_URI = "http://www.boldsystems.org/index.php/Public_RecordView?processid=%s";
|
101
|
|
102
|
@XmlElement( name = "DnaSample")
|
103
|
@XmlIDREF
|
104
|
@XmlSchemaType(name = "IDREF")
|
105
|
@ManyToOne(fetch = FetchType.LAZY)
|
106
|
@IndexedEmbedded
|
107
|
private DnaSample dnaSample;
|
108
|
|
109
|
|
110
|
/** @see #getContigFile() */
|
111
|
@XmlElement(name = "ContigFile")
|
112
|
@XmlIDREF
|
113
|
@XmlSchemaType(name = "IDREF")
|
114
|
@ManyToOne(fetch = FetchType.LAZY)
|
115
|
@Cascade({CascadeType.SAVE_UPDATE,CascadeType.MERGE})
|
116
|
private Media contigFile;
|
117
|
|
118
|
/** @see #getConsensusSequence() */
|
119
|
@XmlElement(name = "ConsensusSequence")
|
120
|
private SequenceString consensusSequence = SequenceString.NewInstance();
|
121
|
|
122
|
@XmlAttribute(name = "isBarcode")
|
123
|
private Boolean isBarcode = null;
|
124
|
|
125
|
/** @see #getBarcodeSequence()*/
|
126
|
@XmlElement(name = "BarcodeSequencePart")
|
127
|
private SequenceString barcodeSequencePart = SequenceString.NewInstance();
|
128
|
|
129
|
/** @see #getGeneticAccessionNumber()*/
|
130
|
@XmlElement(name = "GeneticAccessionNumber")
|
131
|
@Column(length=20)
|
132
|
private String geneticAccessionNumber;
|
133
|
|
134
|
/** @see #getBoldProcessId() */
|
135
|
@XmlElement(name = "BoldProcessId")
|
136
|
@Column(length=20)
|
137
|
private String boldProcessId;
|
138
|
|
139
|
@XmlElementWrapper(name = "SingleReadAlignments")
|
140
|
@XmlElement(name = "SingleReadAlignment")
|
141
|
@XmlIDREF
|
142
|
@XmlSchemaType(name = "IDREF")
|
143
|
@OneToMany(mappedBy="consensusAlignment", fetch = FetchType.LAZY, orphanRemoval=true)
|
144
|
@Cascade({CascadeType.SAVE_UPDATE,CascadeType.MERGE})
|
145
|
private Set<SingleReadAlignment> singleReadAlignments = new HashSet<SingleReadAlignment>();
|
146
|
|
147
|
/** @see #getDnaMarker() */
|
148
|
@XmlElement(name = "DnaMarker")
|
149
|
@XmlIDREF
|
150
|
@XmlSchemaType(name = "IDREF")
|
151
|
@ManyToOne(fetch = FetchType.LAZY)
|
152
|
//no cascade as it is a defined term
|
153
|
private DefinedTerm dnaMarker;
|
154
|
|
155
|
|
156
|
/** @see #getHaplotype() */
|
157
|
@XmlElement(name = "Haplotype")
|
158
|
@Column(length=100)
|
159
|
private String haplotype;
|
160
|
|
161
|
/** @see #getCitations() */
|
162
|
@XmlElementWrapper(name = "Citations")
|
163
|
@XmlElement(name = "Citation")
|
164
|
@XmlIDREF
|
165
|
@XmlSchemaType(name = "IDREF")
|
166
|
@ManyToMany(fetch = FetchType.LAZY)
|
167
|
@Cascade({CascadeType.SAVE_UPDATE,CascadeType.MERGE})
|
168
|
private Set<Reference> citations = new HashSet<>();
|
169
|
|
170
|
// //should be calculated in case sequence is set
|
171
|
// @XmlElement (name = "DateSequenced", type= String.class)
|
172
|
// @XmlJavaTypeAdapter(DateTimeAdapter.class)
|
173
|
// @Type(type="dateTimeUserType")
|
174
|
// @Basic(fetch = FetchType.LAZY)
|
175
|
// private DateTime dateSequenced;
|
176
|
|
177
|
|
178
|
//*********************** FACTORY ****************************************************/
|
179
|
|
180
|
public static Sequence NewInstance(String consensusSequence){
|
181
|
Sequence result = new Sequence();
|
182
|
result.setSequenceString(consensusSequence);
|
183
|
return result;
|
184
|
}
|
185
|
|
186
|
|
187
|
public static Sequence NewInstance(String consensusSequence, Integer length){
|
188
|
Sequence result = NewInstance(consensusSequence);
|
189
|
result.getConsensusSequence().setLength(length);
|
190
|
return result;
|
191
|
}
|
192
|
|
193
|
public static Sequence NewInstance(DnaSample dnaSample, String consensusSequence, Integer length){
|
194
|
Sequence result = NewInstance(consensusSequence);
|
195
|
result.getConsensusSequence().setLength(length);
|
196
|
dnaSample.addSequence(result);
|
197
|
|
198
|
return result;
|
199
|
}
|
200
|
//*********************** CONSTRUCTOR ****************************************************/
|
201
|
|
202
|
protected Sequence() {}
|
203
|
|
204
|
//*********************** GETTER / SETTER ****************************************************/
|
205
|
|
206
|
|
207
|
/**
|
208
|
* The {@link DnaSample dna sample} this sequencing belongs too.
|
209
|
*/
|
210
|
public DnaSample getDnaSample() {
|
211
|
return dnaSample;
|
212
|
}
|
213
|
|
214
|
/**
|
215
|
* To be called only from {@link DnaSample#addSequence(Sequence)}
|
216
|
* @see #getDnaSample()
|
217
|
*/
|
218
|
//TODO implement full bidirectionality
|
219
|
protected void setDnaSample(DnaSample dnaSample) {
|
220
|
this.dnaSample = dnaSample;
|
221
|
if (dnaSample != null && !dnaSample.getSequences().contains(this)){
|
222
|
throw new RuntimeException("Don't use DNA setter");
|
223
|
}
|
224
|
}
|
225
|
|
226
|
/**
|
227
|
* The resulting consensus sequence represened by this {@link Sequence sequence} .
|
228
|
* The consensus is usually computed from the {@link SingleRead single reads}.
|
229
|
* The result of which is stored in a file called {@link #getContigFile() contig file}
|
230
|
*
|
231
|
* #see {@link #getContigFile()}
|
232
|
* #see {@link #getSingleReads()}
|
233
|
*/
|
234
|
public SequenceString getConsensusSequence() {
|
235
|
return consensusSequence;
|
236
|
}
|
237
|
|
238
|
|
239
|
/**
|
240
|
* @see #getConsensusSequence()
|
241
|
*/
|
242
|
public void setConsensusSequence(SequenceString sequenceString) {
|
243
|
if (sequenceString == null){
|
244
|
sequenceString = SequenceString.NewInstance();
|
245
|
}
|
246
|
this.consensusSequence = sequenceString;
|
247
|
}
|
248
|
|
249
|
/**
|
250
|
* The isBarcode flag should be set to true if this (consensus) sequence is or includes
|
251
|
* a barcoding sequence. If the barcoding sequence is only a part of the consensus sequence
|
252
|
* this part shall be stored as {@link #getBarcodeSequencePart() barcoding sequence part}.
|
253
|
* A isBarcode value of <code>null</code> indicates that we do have no knowledge
|
254
|
* whether the sequence is a barcoding sequence or not.
|
255
|
*
|
256
|
* @see #getBarcodeSequencePart()
|
257
|
* @see #getSequenceString()
|
258
|
* @returns the isBarcode flag value (tri-state)
|
259
|
*
|
260
|
*/
|
261
|
public Boolean getIsBarcode() {
|
262
|
return isBarcode;
|
263
|
}
|
264
|
|
265
|
/**
|
266
|
* @see #getIsBarcode()
|
267
|
* @see #getBarcodeSequencePart()
|
268
|
*/
|
269
|
public void setIsBarcode(Boolean isBarcode) {
|
270
|
this.isBarcode = isBarcode;
|
271
|
}
|
272
|
|
273
|
/**
|
274
|
* If the barcode sequence string does not include 100% of the (consensus) sequence
|
275
|
* the part used as barcode is provided here. However, the barcode part
|
276
|
* should be kept empty if consensus sequence string and barcode sequence string are equal.
|
277
|
*
|
278
|
* @see #getIsBarcode()
|
279
|
*/
|
280
|
public SequenceString getBarcodeSequencePart() {
|
281
|
return barcodeSequencePart;
|
282
|
}
|
283
|
|
284
|
/**
|
285
|
* @see #getBarcodeSequencePart()
|
286
|
*/
|
287
|
public void setBarcodeSequencePart(SequenceString barcodeSequencePart) {
|
288
|
if (barcodeSequencePart == null){
|
289
|
barcodeSequencePart = SequenceString.NewInstance();
|
290
|
}
|
291
|
this.barcodeSequencePart = barcodeSequencePart;
|
292
|
}
|
293
|
|
294
|
/**
|
295
|
* Sets the {@link TermType#DnaMarker DNA marker} examined and described by this sequencing.
|
296
|
* The marker should usually be similar to the one used in the according {@link Amplification
|
297
|
* amplification process}. However, it may slightly differ, or, if multiple amplifications where
|
298
|
* used to build this consensus sequence it may be the super set of the markers used in amplification.
|
299
|
*
|
300
|
* @return
|
301
|
*/
|
302
|
public DefinedTerm getDnaMarker(){
|
303
|
return this.dnaMarker;
|
304
|
}
|
305
|
|
306
|
/**
|
307
|
* @see #getDnaMarker()
|
308
|
* @param marker
|
309
|
*/
|
310
|
public void setDnaMarker(DefinedTerm dnaMarker){
|
311
|
this.dnaMarker = dnaMarker;
|
312
|
}
|
313
|
|
314
|
/**
|
315
|
* The accession number used in GenBank, EMBL and DDBJ.
|
316
|
* @return
|
317
|
*/
|
318
|
public String getGeneticAccessionNumber() {
|
319
|
return geneticAccessionNumber;
|
320
|
}
|
321
|
|
322
|
/**
|
323
|
* Sets the genetic accession number.
|
324
|
* @see #getGeneticAccessionNumber()
|
325
|
*/
|
326
|
public void setGeneticAccessionNumber(String geneticAccessionNumber) {
|
327
|
this.geneticAccessionNumber = geneticAccessionNumber;
|
328
|
}
|
329
|
|
330
|
|
331
|
/**
|
332
|
* The identifier used by the Barcode of Life Data Systems (BOLD, http://www.boldsystems.org/).
|
333
|
*/
|
334
|
public String getBoldProcessId() {
|
335
|
return boldProcessId;
|
336
|
}
|
337
|
|
338
|
public void setBoldProcessId(String boldProcessId) {
|
339
|
this.boldProcessId = boldProcessId;
|
340
|
}
|
341
|
|
342
|
/**
|
343
|
* Returns the name of the haplotype.
|
344
|
* A haplotype (haploide genotype) is a variant of nucleotide sequences on the same chromosome.
|
345
|
* A certain haplotype may be specific for an individual, a population or a species.
|
346
|
* @return
|
347
|
*/
|
348
|
public String getHaplotype() {
|
349
|
return haplotype;
|
350
|
}
|
351
|
|
352
|
/**
|
353
|
* @see #getHaplotype()
|
354
|
*/
|
355
|
public void setHaplotype(String haplotype) {
|
356
|
this.haplotype = haplotype;
|
357
|
}
|
358
|
|
359
|
/**
|
360
|
* The contigFile containing all data and data processing for this sequencing.
|
361
|
*
|
362
|
* @see #getConsensusSequence()
|
363
|
* @see #getSingleReads()
|
364
|
*/
|
365
|
public Media getContigFile() {
|
366
|
return contigFile;
|
367
|
}
|
368
|
|
369
|
/**
|
370
|
* @see #getContigFile()
|
371
|
*/
|
372
|
public void setContigFile(Media contigFile) {
|
373
|
this.contigFile = contigFile;
|
374
|
}
|
375
|
|
376
|
|
377
|
/**
|
378
|
* Citations are the set of references in which this sequence was published.
|
379
|
* Unlike taxonomic names the first publication of a sequence
|
380
|
* is not so important (maybe because it is required by publishers
|
381
|
* that they are all registered at Genbank) therefore we do not have something like an
|
382
|
* "original reference" attribute.<BR>
|
383
|
* Links to these references are to be stored within the reference itself.
|
384
|
* @return the set of references in which this sequence was published.
|
385
|
*/
|
386
|
public Set<Reference> getCitations() {
|
387
|
return citations;
|
388
|
}
|
389
|
/**
|
390
|
* @see #getCitations()
|
391
|
*/
|
392
|
protected void setCitations(Set<Reference> citations) {
|
393
|
this.citations = citations;
|
394
|
}
|
395
|
/**
|
396
|
* @see #getCitations()
|
397
|
*/
|
398
|
public void addCitation(Reference citation) {
|
399
|
this.citations.add(citation);
|
400
|
}
|
401
|
/**
|
402
|
* @see #getCitations()
|
403
|
*/
|
404
|
public void removeCitation(Reference citation) {
|
405
|
this.citations.remove(citation);
|
406
|
}
|
407
|
|
408
|
/**
|
409
|
* The {@link SingleRead single reads} that were used to build this consensus sequence.
|
410
|
*
|
411
|
* @see #getConsensusSequence()
|
412
|
* @see #getContigFile()
|
413
|
*/
|
414
|
public Set<SingleReadAlignment> getSingleReadAlignments() {
|
415
|
return singleReadAlignments;
|
416
|
}
|
417
|
/**
|
418
|
* @see #getSingleReads()
|
419
|
*/
|
420
|
public void addSingleReadAlignment(SingleReadAlignment singleReadAlignment) {
|
421
|
this.singleReadAlignments.add(singleReadAlignment);
|
422
|
if (! this.equals(singleReadAlignment.getConsensusSequence())){
|
423
|
singleReadAlignment.setConsensusAlignment(this);
|
424
|
};
|
425
|
}
|
426
|
/**
|
427
|
* @see #getSingleReads()
|
428
|
*/
|
429
|
public void removeSingleReadAlignment(SingleReadAlignment singleReadAlignment) {
|
430
|
this.singleReadAlignments.remove(singleReadAlignment);
|
431
|
if (this.equals(singleReadAlignment.getConsensusSequence())){
|
432
|
singleReadAlignment.setConsensusAlignment(null);
|
433
|
singleReadAlignment.setSingleRead(null);
|
434
|
}
|
435
|
}
|
436
|
// /**
|
437
|
// * @see #getSingleReads()
|
438
|
// */
|
439
|
// //TODO private as long it is unclear how bidirectionality is handled
|
440
|
// @SuppressWarnings("unused")
|
441
|
// private void setSingleReadAlignments(Set<SingleReadAlignment> singleReadAlignments) {
|
442
|
// this.singleReadAlignments = singleReadAlignments;
|
443
|
// }
|
444
|
|
445
|
// *********************** CONVENIENCE ***********************************/
|
446
|
|
447
|
/**
|
448
|
* Convenience method to add a single read to a consensus sequence
|
449
|
* by creating a {@link SingleReadAlignment}.
|
450
|
* @param singleRead the {@link SingleRead} to add
|
451
|
* @return the created SingleReadAlignment
|
452
|
*/
|
453
|
public SingleReadAlignment addSingleRead(SingleRead singleRead) {
|
454
|
SingleReadAlignment alignment = SingleReadAlignment.NewInstance(this, singleRead);
|
455
|
return alignment;
|
456
|
}
|
457
|
|
458
|
public void removeSingleRead(SingleRead singleRead) {
|
459
|
Set<SingleReadAlignment> toRemove = new HashSet<SingleReadAlignment>();
|
460
|
for (SingleReadAlignment align : this.singleReadAlignments){
|
461
|
if (align.getSingleRead() != null && align.getSingleRead().equals(singleRead)){
|
462
|
toRemove.add(align);
|
463
|
}
|
464
|
}
|
465
|
for (SingleReadAlignment align : toRemove){
|
466
|
removeSingleReadAlignment(align);
|
467
|
}
|
468
|
return;
|
469
|
}
|
470
|
|
471
|
/**
|
472
|
* Convenience method that returns all single reads this consensus sequence
|
473
|
* is based on via {@link SingleReadAlignment}s.
|
474
|
* @return set of related single reads
|
475
|
*/
|
476
|
@XmlTransient
|
477
|
@Transient
|
478
|
public Set<SingleRead> getSingleReads(){
|
479
|
Set<SingleRead> singleReads = new HashSet<SingleRead>();
|
480
|
for (SingleReadAlignment align : this.singleReadAlignments){
|
481
|
if (align.getSingleRead() != null){ // == null should not happen
|
482
|
singleReads.add(align.getSingleRead());
|
483
|
}
|
484
|
}
|
485
|
return singleReads;
|
486
|
}
|
487
|
|
488
|
|
489
|
//*************************** Transient GETTER /SETTER *****************************/
|
490
|
|
491
|
/**
|
492
|
* Delegate method to get the text representation of the consensus sequence
|
493
|
* @see #setSequenceString(String)
|
494
|
*/
|
495
|
@Transient
|
496
|
public String getSequenceString() {
|
497
|
return consensusSequence.getString();
|
498
|
}
|
499
|
|
500
|
/**
|
501
|
* Delegate method to set the text representation of the {@link #getConsensusSequence()
|
502
|
* consensus sequence}.
|
503
|
*/
|
504
|
@Transient
|
505
|
public void setSequenceString(String sequence) {
|
506
|
consensusSequence.setString(sequence);
|
507
|
}
|
508
|
|
509
|
/**
|
510
|
* Convenience method which computes the set of all related pherograms
|
511
|
* @return the set of pherograms.
|
512
|
*/
|
513
|
@Transient
|
514
|
public Set<Media> getPherograms(){
|
515
|
Set<Media> result = new HashSet<Media>();
|
516
|
for (SingleReadAlignment singleReadAlign : singleReadAlignments){
|
517
|
if (singleReadAlign.getSingleRead() != null && singleReadAlign.getSingleRead().getPherogram() != null){
|
518
|
result.add(singleReadAlign.getSingleRead().getPherogram());
|
519
|
}
|
520
|
}
|
521
|
return result;
|
522
|
}
|
523
|
|
524
|
|
525
|
//***** Registrations ************/
|
526
|
/**
|
527
|
* Returns the computed genBank uri.
|
528
|
* @return the uri composed of {@link #GENBANK_BASE_URI} and {@link #geneticAccessionNumber}
|
529
|
* @throws URISyntaxException when URI could not be created with {@link #geneticAccessionNumber}
|
530
|
*/
|
531
|
@Transient
|
532
|
public URI getGenBankUri() throws URISyntaxException {
|
533
|
return createExternalUri(GENBANK_BASE_URI, geneticAccessionNumber);
|
534
|
}
|
535
|
|
536
|
/**
|
537
|
* Returns the computed EMBL uri.
|
538
|
* @return the uri composed of {@link #EMBL_BASE_URI} and {@link #geneticAccessionNumber}
|
539
|
* @throws URISyntaxException when URI could not be created with {@link #geneticAccessionNumber}
|
540
|
*/
|
541
|
@Transient
|
542
|
public URI getEmblUri() throws URISyntaxException {
|
543
|
return createExternalUri(EMBL_BASE_URI, geneticAccessionNumber);
|
544
|
}
|
545
|
|
546
|
/**
|
547
|
* Returns the computed DDBJ uri.
|
548
|
* @return the uri composed of {@link #DDBJ_BASE_URI} and {@link #geneticAccessionNumber}
|
549
|
* @throws URISyntaxException when URI could not be created with {@link #geneticAccessionNumber}
|
550
|
*/
|
551
|
@Transient
|
552
|
public URI getDdbjUri() throws URISyntaxException {
|
553
|
return createExternalUri(DDBJ_BASE_URI, geneticAccessionNumber);
|
554
|
}
|
555
|
|
556
|
/**
|
557
|
* Returns the URI for the BOLD entry.
|
558
|
* @return the uri composed of {@link #BOLD_BASE_URI} and {@link #boldProcessId}
|
559
|
* @throws URISyntaxException when URI could not be created with {@link #boldProcessId}
|
560
|
* @see #getBoldProcessId()
|
561
|
*/
|
562
|
@Transient
|
563
|
public URI getBoldUri() throws URISyntaxException {
|
564
|
return createExternalUri(BOLD_BASE_URI, boldProcessId);
|
565
|
}
|
566
|
|
567
|
private URI createExternalUri(String baseUri, String id) throws URISyntaxException{
|
568
|
if (CdmUtils.isNotBlank(id)){
|
569
|
return new URI(String.format(baseUri, id.trim()));
|
570
|
}else{
|
571
|
return null;
|
572
|
}
|
573
|
}
|
574
|
|
575
|
|
576
|
|
577
|
|
578
|
//*********************** CLONE ********************************************************/
|
579
|
/**
|
580
|
* Clones <i>this</i> sequence. This is a shortcut that enables to create
|
581
|
* a new instance that differs only slightly from <i>this</i> sequencing by
|
582
|
* modifying only some of the attributes.<BR><BR>
|
583
|
*
|
584
|
*
|
585
|
* @see eu.etaxonomy.cdm.model.media.IdentifiableEntity#clone()
|
586
|
* @see java.lang.Object#clone()
|
587
|
*/
|
588
|
@Override
|
589
|
public Object clone() {
|
590
|
try{
|
591
|
Sequence result = (Sequence)super.clone();
|
592
|
|
593
|
//sequences
|
594
|
result.consensusSequence = (SequenceString)this.consensusSequence.clone();
|
595
|
result.barcodeSequencePart = (SequenceString)this.barcodeSequencePart.clone();
|
596
|
|
597
|
|
598
|
//single sequences
|
599
|
result.singleReadAlignments = new HashSet<SingleReadAlignment>();
|
600
|
for (SingleReadAlignment singleReadAlign: this.singleReadAlignments){
|
601
|
SingleReadAlignment newAlignment = (SingleReadAlignment)singleReadAlign.clone();
|
602
|
result.singleReadAlignments.add(newAlignment);
|
603
|
}
|
604
|
|
605
|
//citations //TODO do we really want to copy these ??
|
606
|
result.citations = new HashSet<Reference>();
|
607
|
for (Reference ref: this.citations){
|
608
|
result.citations.add(ref);
|
609
|
}
|
610
|
|
611
|
|
612
|
|
613
|
return result;
|
614
|
}catch (CloneNotSupportedException e) {
|
615
|
logger.warn("Object does not implement cloneable");
|
616
|
e.printStackTrace();
|
617
|
return null;
|
618
|
}
|
619
|
}
|
620
|
|
621
|
|
622
|
}
|